Intracellular pH of sea urchin eggs measured by the dimethyloxazolidinedione (DMO) method.
نویسندگان
چکیده
منابع مشابه
Intracellular pH of sea urchin eggs measured by the dimethyloxazolidinedione (DMO) method
Intracellular pH (pH1) of sea urchin eggs and embryos was determined using DMO (5,5-dimethyl-2,4-oxazolidinedione). By this method, the pH1 of Lytechinus pictus eggs increased after fertilization from 6.86 to 7.27, and this higher pHi was maintained thereafter, as has been previously observed with pH microelectrodes. The same general result was obtained with the eggs of Strongylocentrotus purpu...
متن کاملRegulation of intracellular pH in sea urchin eggs by medium containing both weak acid and base.
To establish a method of pHi regulation and to understand the pH regulation mechanism in the cell, we investigated the pHi response of unfertilized or fertilized eggs of sea urchin, applying sea water containing both weak permeant acid, acetic acid and/or base, ammonia, to eggs. Pyranine was employed as a pH indicator to measure intracellular pH (pHi) by microfluorometry. The unfertilized/ferti...
متن کاملAction of Colcemid in Sea Urchin Eggs
The effects of Colcemid, the deacetyl-N-methyl derivative of colchicine, on the eggs of Arbacia punctulata were investigated. Colcemid in concentrations of 2.7 x 10(-5)M or greater blocks syngamy (the fusion of the pronuclei) in these eggs. Although a tenfold decrease in concentration of Colcemid usually permits the pronuclei to fuse, the subsequent division is blocked. In the sea urchin egg, t...
متن کاملElectrophoretic movement of fertilized sea-urchin eggs.
The eccentric shift of the sea-urchin egg within the fertilization membrane under an electric field was analysed by measuring the electrophoretic mobility of the isolated fertilization membrane and that of the egg deprived of the fertilization membrane. In addition, the migration speed of the egg proper was measured within the fertilization membrane under the conditions that: either (1) the mov...
متن کاملIsolation of cortical material from sea urchin eggs by centrifugation.
Although the surface layers of the sea urchin egg are the site of dramatic changes at fertilization, little is actually known about the submicroscopic structure of the surface layers, their chemistry, or the real nature of the physiological changes that are collectively referred to as the cort ical reac tiom. Excluding the jelly, the surface layers of the unfertilized egg that have been recogni...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1981
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.89.2.284